Caprine Health

5th Ed. Vet Clinical Parasitology Reprint Participation Requested
DNA Procedure Using Hair Sample
From: Sue Reith
Sent: Sunday, December 14, 2003 6:39 PM
 

I'm turning to you all for support in getting a reprint of those wonderful parasite eggs/oocysts (99 straight sequential pages of great photos, covering internal parasite eggs/oocysts from farm animals, domestic birds, pets, and some wildlife) found in the 1st thru 5th Editions of Sloss' Vet Clinical Parasitology but dropped from the 6th Edition.

Many people have thoughtfully suggested as a substitute some currently available books that they hoped might provide the same level of help in ID'ing what's being viewed on the slide. But I think it's possible that until someone actually works with the Sloss photos to ID eggs/oocysts on a slide it'd be hard to grasp the full impact of the superior ease and level of ID accuracy they afford the viewer. The cold, hard fact remains that accuracy in ID of eggs/oocysts is the key to successful parasite extermination... And while the alternatively suggested books are all very educational and informative, frankly none of them come close to the Sloss photos for that specific, crucial purpose.

As an example, one book suggested has been Foreyt's Veterinary Parasitology Ed.4, and now Ed.5 out as well... There's no denying that this book is informative and educational, but it doesn't come close to the quality of reference provided by the Sloss pics. This idea Margaret Sloss had to devote a whole page to each worm egg, with a photo of an egg cluster at the top of the page so the viewer can detect minor natural variations within eggs of the same species, and then a big, sharp close-up of the individual egg at the bottom of the page for fine detail, is such an ingeniously helpful presentation! And additionally, using the goat/sheep host species as an example, the whole range of 12 eggs/oocysts in the series commonly found in our goats/sheep is neatly grouped together into 6 pages so the viewer can put it right there beside the scope and flip quickly for fast, easy, efficient reference while working! It's so cool! In these other recommended books some of the egg representations are hand drawn, others are 'generic', and more than a few of the representations are just plain awful pics! And yes, the pics of eggs common to a specific host that are purported to be grouped by species together actually are grouped, sort of, but then they are scattered at random all over the place within that grouping, with one found on one page, and another several pages away, admittedly with some good info about history, habits, mode of extermination, et al, in-between one pic and the next, which is nice when studying about worms in general, but it's terribly inefficient, not to mention distracting, when you have but one focus, that of a clear, concise ID of what's on that slide! The Sloss photo setup enables the viewer to quickly check just those few sequential pages where you know what you're looking for is going to be found, which is incredibly easy, quick and efficient.

One of the of the rapidly-growing number of animal owners/caregivers who are taking charge of their own animals' parasite control has reached out and wrtten an email to the Director of the Iowa State Univ Press. The delightful result of her effort has been that the author of the forthcoming 7th Ed. responded with the following request:

Anne Zajac DVM, PhD, azajac@vt.edu, who is at the moment preparing the manuscript for the forthcoming 7th Edition of Veterinary Clinical Parasitology for the Iowa State Univ Press writes:

>>You say in your email that older editions are superior for several reasons to the current one. I was wondering if you might tell me what those reasons are? The new edition is nearly finished, but I am always interested in how we can improve the book for its users.<<

What a wonderful opportunity for us! In my view it makes no difference whether those ID photos Dr Sloss created become available in a reprint of the 5th Edition, or whether they become a part of the forthcoming 7th Edition! The 5th Edition, BTW, wasn't just about the 99 wonderful pages of excellent egg/oocyst photos... It also had good information and photos on external parasites, etc, and on other forms of slide prep for such things as blood work, et al... And I'm confident that Dr Zajac will do a good job on the upcoming 7th Edition as well... So long as it has those great photos incorporated into it, with the same format previously used, for our benefit.

When I wrote my own response to Dr Zajac's letter above, for even more 'clout' I sent Cc's: to marketing@iowastatepress.com; director@iowastatepress.com; and anderson@iowastatepress.com; all 3 of whom head up departments at the Iowa State Univ Press. Maybe you've heard that the squeaking wheel gets oiled? (big smile)

So I urge all of you who've been unable to get a book containing those pics yourselves, many of whom have asked me to send you my post containing the ones common to goats/sheep, to write to Dr Zajac and tell her just why we need those photos. And I feel it's especially appropriate for you to contact her with your concerns if you are among those that have asked for my help because you wanted to be able to do fecals on horses, cats, dogs, ducks, wildlife, or any of the other species beyond the goats/sheep pics I have been able to provide, or if you know other people that could use that info.

And finally, I would greatly appreciate people letting me know when they follow thru on this... It's been very helpful to have had so many write already to share their efforts... And it has been especially rewarding that several have provided exciting options to follow up on if we can't get Iowa State Univ Press to do this for us all, either in a reprint or in the coming 7th Edition of the book. It's great to have options if the original goal can't be achieved, although in my view it would be incredibly preferable for the original publisher to handle the job...

 

Sue Reith
Carmelita Toggs
Bainbridge Island WA
suereith@msn.com

1. Pluck between 15 and 20 hair strands from the animal, making sure that you grab the hairs near the base. From the tail switch or under the mane, grab the hairs near their base.

2. Quickly pull the hairs out, and then inspect them closely to make sure the roots are intact. DNA is extracted from the hair bulb. The bulb must be preserved; pulling is the best way to do this. DO NOT cut the hair.

3. Once you have the strands of hair, place them in a zip-loc bag. Use a separate bag for each animal.

4. Place the plastic bag(s) into an executive size envelope with the identification form(s).

5. Hair can be stored in a resealable bag almost indefinitely and may be shipped by U.S. Postal Service 3rd Class or other courier. There is no need to overnight the samples. Ship to DNA Lab at University closest to you.


Gentovin, Garacin

Do not use in food producing animals

50mg/ml or 100mg/ml

DOSE : Given SC (SQ) at <1 to 1.50mg/lb twice daily. Often combined with penicillin to achieve a broad spectrum of activity and to minize the development of resistant strains. When gentamicin is administered together with penicillin, the two, medicines must be administered at different sites. NOTE: Dose carefully in obese animals (decrease by 10- 20% in very obese animals), the aminoglycosides do not distribute to fat.

Ophthalmic Solution: (3 mg/ml) - Mix 1ml if 50mg/ml strength in 16ml Saline Solution [or commercial eye drops i.e. Visine] and use to treat infected eyes. Ophthalmic use may retard corneal epithelial healing.

WITHDRAWAL : Not approved for meat or lactating animals in the US. Can be detected in kidney tissues for up to 18 months.

Use with carebacterial resistance to gentamicin is developing .

CAUTION: Before using always check the label for the strength, gentamicin comes in several strengths and administering a 50mg dose of 100mg gentamicin could cause serious damage.

CAUTION: Gentamicin must be carefully dosed by the weight of the animal [weigh chart ] and for a limited time (3-5 days), as it is highly toxic to the kidneys (acute tubular necrosis) and ears ( irreversible acoustic nerve damage ) when overdosed even a relitively small amount or used for extended periods of time, particularly in dehydrated, debilitated or young animals. Avoid in geriatric animals.

Do not use with diuretics as it increases the risk of kidney damage.

Do not use in combination with other aminoglycosides.

Do not use with other products that may cause hearing loss, vestibular disease, or kidney disease.

Do not use with general anesthetics.

CAUTION : GENTAMICIN SHOULD NEVER BE USED IN FOOD PRODUCING ANIMALS OR ANIMALS INTENDED FOR FOOD (residues can be detected in kidney tissues for up to 18 months).


 

 

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